David Rueda

The Single-Molecule Imaging Group develops and applies single-molecule imaging approaches to study how structural dynamics regulate fundamental biological processes involving proteins and nucleic acids across scales (from molecules to cells). We are particularly interested in elucidating various mechanistic aspects of DNA and RNA processing, such as chromatin structure and remodelling, DNA replication and repair, and RNA transcription, splicing and localization. Single-Molecule Microscopy reveals the structural dynamics of individual molecules, otherwise hidden in ensemble-averaged experiments, thus enabling us to directly observe key reaction intermediates, even when short-lived or at low levels. We currently focus on four main areas.

Publications

Journals

• Aristodemou AEN, Rueda DS, Taylor GP, et al., 2023, The transcriptome of HTLV-1-infected primary cells following reactivation reveals changes to host gene expression central to the proviral life cycle
• Belan O, Sebald M, Adamowicz M, et al., 2022, POLQ seals post-replicative ssDNA gaps to maintain genome stability in BRCA-deficient cancer cells., Mol Cell, Vol:82, Pages:4664-4680.e9
• Moore G, Han Z, Xu J, et al., 2022, Dynamic Backtracking Regulates Lesion Bypass by RNAPII
• Kaczmarczyk AP, Déclais A-C, Newton MD, et al., 2022, Search and processing of Holliday junctions within long DNA by junction-resolving enzymes, Nature Communications, Vol:13, Pages:1-13

Affiliations

• Centre for Advanced Therapeutics
• Frontiers of Ultrafast Measurement
• Innovative Imaging Across Scales Network
• Molecular Sciences
• Section of Virology

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